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Abstract

Temephos is extensively employed currently to eliminate Aedes aegypti mosquito larvae, thus preventing the spread of dengue fever. Raising the level of temephos application can lead to health issues for individuals and long-term detrimental impacts on the environment. This research aims to demonstrate that Z. purpureum Rosc. essential oil effective in eliminating more than 70% of Aedes aegypti mosquito larvae. Z. purpureum Rosc.essential oil was diluted with Tween 80 to achieve various concentrations, namely 12.5 μg/mL, 25 μg/mL, 50 μg/mL, and 100 μg/mL. A positive control was established using temephos at a concentration of 0.012 mg/L, while a negative control was prepared using clean water. The larvae were subjected to these treatments for a duration of 48 hours, with three repetitions conducted. The data were analyzed using the Kruskal Wallis Test that shows a significant difference between treatment groups, with a p value <0.05. A concentration of 12.5 μg/mL of Z. purpureum Rosc. essential oil was found to effectively eliminate 76.67% of Aedes aegypti mosquito larvae. A concentration of 25 μg/mL of Z. purpureum Rosc. essential oil was found to effectively eliminate 86.67% of Aedes aegypti mosquito larvae. A concentration of 50 μg/mL of Z. purpureum Rosc. essential oil was found to effectively eliminate 96.67% of Aedes aegypti mosquito larvae. A concentration of 100 μg/mL of Z. purpureum Rosc. essential oil was found to effectively eliminate 100% of Aedes aegypti mosquito larvae. The overall findings show that more than 70% of Aedes aegypti mosquito larvae are effectively killed by Z. purpureum Rosc. essential oil at a concentration of 12.5 μg/mL.

Article Details

How to Cite
Syahdumawarta, L. I., Toruan, V. M. L., Paramita, S., & Zubaidah, M. (2024). Larvicidal Activity of Zingiber purpureum Roscoe Essential Oil from East Kalimantan in Preventing Dengue Hemorrhagic Fever. International Journal of Multidisciplinary: Applied Business and Education Research, 5(9), 3609-3614. https://doi.org/10.11594/ijmaber.05.09.20

References

Abdelrahman, M., & Jogaiah, S. (2020). Bioac-tive molecules in plant defense. In
Bioactive molecules in plant defense. Springer International Publishing.
Armyandi, A., Wahyuni, D., & Kamalia, F. (2022). Toksisitas ekstrak terpurifikasi dengan n-heksan buah kecubung (Datura metel L.) terhadap mortalitas larva nya-muk Aedes aegypti. Saintifika, 24(1), 55-67.
Artini, P. E. U. D. (2013). Uji fitokimia etil asetat rimpang bangle. Jurnal Kefarma-sian, Udayana University.
Baharun, K., Rukmi, I., Lunggani, A. T., & Fachriyah, E. (2013). Rimpang temu hitam (Curcuma aeruginosa Roxb.) ter-hadap Bacillus subtilis dan Staphylococ-cus aureus secara in vitro. Jurnal Biologi, 2(24), 16-24.
Devkota, H. P., Paudel, K. R., Hassan, M. M., Dirar, A. I., Das, N., Devkota, A. A., ... & Dua, K. (2021). Bioactive compounds from Zingiber montanum and their pharmacological activities with focus on zerumbone. Applied Sciences, 11(21).
Dewi, D. P., Wahyuni, & Suratno. (2014). Tok-sisitas granula ekstrak biji alpukat (Per-sea americana Mill) terhadap mortalitas larva nyamuk Aedes aegypti. Artikel Ilmiah, 1-5.
Dinkes NTB. (2017). Obat pembunuh jentik nyamuk (Abate). Datin Litbangkes. https://dinkes.ntbprov.go.id/artikel/obat-pembunuh-jentik-nyamuk-abate/
Directorate of Fertilizer and Pesticide. (2012). Metode standar pengujian efikasi insek-tisida. Ministry of Agriculture.
Du, J. R., Long, F. Y., & Chen, C. (2014). Re-search progress on natural triterpenoid saponins in the chemoprevention and chemotherapy of cancer. Enzymes, 36, 95–130.
Funayama, S., & Cordell, G. A. (2014). Alka-loids: A treasury of poisons and medi-cines. Alkaloids: A Treasury of Poisons and Medicines, 1–284.
Huong, L. T. (2020). Chemical composition and larvicidal activity of essential oils from Zingiber montanum (J. Koenig) Link ex. Dietr. against three mosquito vectors. Bo-letin Latinoamericano y Del Caribe de Plantas Medicinales y Aromáticas, 57, 1-10.
Idris, H. (2015). Tanaman kecubung (Datura metel L.) sebagai bahan baku insektisida botanis untuk mengendalikan hama As-pidomorpha miliaris F. Jurnal Littri, 21(1), 41-46.
Indrawati, E. T., Arung, E. T., & Irawan, W. K. (2011). Analisis fitokimia dari beberapa tumbuhan hutan dari Kebun Raya Balik-papan Provinsi Kalimantan Timur. Pro-siding Seminar Nasional, 290-293.
Istiana, H., Farida, H., & Isnaini. (2012). Re-sistance status of Aedes aegypti larvae to temephos in West Banjarmasin. Jurnal BUSKI, 4(2), 53-58.
Izzah, N., Kadang, & Permatasari. (2019). Uji identifikasi senyawa alkaloid ekstrak metanol daun kelor (Moringa oleifera Lamk) dari Kabupaten Ende Nusa Tenggara Timur secara kromatografi la-pis tipis. Jurnal Farmasi Sandi Karsa, 5(1), 52-55.
Juang, Y. P., & Liang, P. H. (2020). Biological and pharmacological effects of synthetic saponins. Molecules, 25(21), 4974.
Ludwiczuk, A., Skalicka-Woźniak, K., & Georgiev, M. I. (2017). Terpenoids. In Pharmacognosy: Fundamentals, Applica-tions and Strategy (pp. 233–266).
Minarni, E., Armansyah, T., & Hanafiah, M. (2013). Daya larvasida ekstrak etil asetat daun kemuning (Murraya paniculata (L) Jack) terhadap larva nyamuk Aedes ae-gypti. Jurnal Medika Veterinaria, 7(1), 27–29.
Paju, N., Yamlean, P. V. Y., & Kojong, N. (2013). Uji efektivitas salep ekstrak daun bi-nahong (Anredera cordifolia (Ten.) Stee-nis) pada kelinci (Oryctolagus cuniculus) yang terinfeksi bakteri Staphylococcus aureus. Pharmacon, 12(1), 51-61.
Pardosi, D. R. S., Purnamasari, C. B., Paramita, S., Astuti, L. A., Masyhudi, M., & Arung, E. T. (2022). Antibacterial activity of bangle rhizoma essential oil (Zingiber monta-num) against Streptococcus mutans. Journal of Dento Maxillofacial Science, 7(1), 5-8.
Prakoso, G., Aulung, A., & Citrawati, M. (2017). Uji efektivitas ekstrak buah pare (Momordica charantia) pada mortalitas larva Aedes aegypti. Jurnal Profesi Medi-ka: Jurnal Kedokteran dan Kesehatan, 10(1).
Pratiwi, A. (2013). Studi deskriptif penerimaan masyarakat terhadap larvasida alami. Thesis, Semarang State University.
Schaefer, T. J., Panda, P. K., & Wolford, R. W. (2022). Dengue fever. StatPearls. https://www.ncbi.nlm.nih.gov/books/NBK430732/
Suparyati, S. (2020). Uji daya bunuh abate berdasarkan dosis dan waktu terhadap kematian larva nyamuk Aedes sp. dan Cu-lex sp. Pena Jurnal Ilmu Pengetahuan Dan Teknologi, 34(2), 1.
Tandi, E. (2010). Pengaruh tanin terhadap ak-tivitas enzim protease. Journal of Science.
Yang, S., Bai, M., Yang, J., Yuan, Y., Zhang, Y., Qin, J., & Sampietro, D. A. (2020). Chemi-cal composition and larvicidal activity of essential oils from Peganum harmala, Nepeta cataria, and Phellodendron amurense against Aedes aegypti (Diptera: Culicidae). Saudi Pharmaceutical Journal, 28(5), 560–564.
Yassi, M. R., & Harsanti. (2018). Uji daya larva-sida ekstrak daun kelor (Moringa oleif-era) terhadap mortalitas larva Aedes ae-gypti. Journal of Agromedicine and Medi-cal Sciences, 4(3), 159-163.